c24 lpc  (GE Healthcare)

Journal: PLoS ONE

Article Title: Phospholipase D Toxins of Brown Spider Venom Convert Lysophosphatidylcholine and Sphingomyelin to Cyclic Phosphates

doi: 10.1371/journal.pone.0072372

Figure Lengend Snippet: Relative concentrations of 8∶0 LPC substrate (squares) and 8∶0 CPA product (circles) as a function of time at 298 K were measured by integration of 31 P-NMR signals relative to a trimethyl phosphate standard (see Materials and Methods), and reported as percentages of the LPC concentration at time zero. Data points for the recombinant enzyme αIB2bi were derived from the experiment shown in , with the H47N variant shown as a negative control. Data for the three venoms were derived from similar experiments, but with addition of whole venom rather than purified recombinant enzyme.

Article Snippet: Natural (purified from chicken egg) and synthetic versions of LPC and SM were purchased from Avanti Polar Lpids (Alabaster, Alabama, USA).

Techniques: Concentration Assay, Recombinant, Derivative Assay, Variant Assay, Negative Control, Purification

Journal: PLoS ONE

Article Title: Phospholipase D Toxins of Brown Spider Venom Convert Lysophosphatidylcholine and Sphingomyelin to Cyclic Phosphates

doi: 10.1371/journal.pone.0072372

Figure Lengend Snippet: (A) Sphingomyelin (SM) is not converted not to ceramide-1-phosphate (C1P) but to a cyclic six membered ceramide(1,3)phosphate [CC(1,3)P]. (B) 31 P-NMR spectra of 4 mM hexanoyl SM (HexSM) micelles, before (top) and 30 min after (bottom) addition of L. arizonica venom. The cyclic product persisted for over 12 h, and no monoester product (i.e. C1P) was detected. Analogous results were observed with purified SicTox αIB2bi enzyme. (C) LC-MS characterization of the product from the NMR sample in (B), showing results analogous to those shown in for conversion of LPC to CPA.

Article Snippet: Natural (purified from chicken egg) and synthetic versions of LPC and SM were purchased from Avanti Polar Lpids (Alabaster, Alabama, USA).

Techniques: Purification, Liquid Chromatography with Mass Spectroscopy

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Single Lysophosphatidylcholine Components Exhibit Adjuvant Activities In Vitro and In Vivo ▿

doi: 10.1128/CVI.00420-09

Figure Lengend Snippet: Mature DC generation induced by LPC single components. The phenotypes of day 6 MoDCs stimulated for 24 h with LPC components (A and B) or LPS (C) were analyzed by flow cytometry. The expression of surface markers on treated cells (thin lines) was compared to that on control cells receiving only PBS (thick lines). The results are representative of those of 1 of 10 experiments.

Article Snippet: l -alpha-Lysophosphatidylcholines (LPCs) from chicken eggs or soybeans and synthetic LPC components with a defined fatty acid esterified at the sn -1 position (lauric acid, C12:0; myristic acid, C14:0; palmitic acid, C16:0; stearic acid, C18:0; oleic acid, C18:1) were purchased from Avanti Polar Lipids (Alabaster, AL).

Techniques: Flow Cytometry, Expressing

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Single Lysophosphatidylcholine Components Exhibit Adjuvant Activities In Vitro and In Vivo ▿

doi: 10.1128/CVI.00420-09

Figure Lengend Snippet: Chemokines secreted by MoDCs upon stimulation with various LPCs. The supernatants of MoDCs treated with LPC components or PBS (A to D) or with LPS (E) at day 5 for 24 h were collected, and the chemokine concentrations were measured by using the CBA technology. Raw data were normalized to the results for the control (PBS treated), which was arbitrarily set equal to 1. The data for each donor are plotted individually; data for a total of seven donors were assayed independently, and the mean for the donors is indicated by the thick horizontal lines. (A) MIP-1β; (B) MCP-1; (C) IL-8; (D) IP-10. The mean values obtained with LPS-treated cells are indicated separately (E).

Article Snippet: l -alpha-Lysophosphatidylcholines (LPCs) from chicken eggs or soybeans and synthetic LPC components with a defined fatty acid esterified at the sn -1 position (lauric acid, C12:0; myristic acid, C14:0; palmitic acid, C16:0; stearic acid, C18:0; oleic acid, C18:1) were purchased from Avanti Polar Lipids (Alabaster, AL).

Techniques: